Input files may be either comma- or tab-separated text files (.csv or .tsv). For more information about the input format, click "Import data file" and make a selection.
For an example input file, click "Load Example" and then "Download Data File" after the file has been loaded.
To calculate normalized growth rate inhibition (GR) values and corresponding GR metrics: GR50, GEC50, GRmax, GRinf, GRAOC, and hGR based on cell counts measured in dose-response experiments using this online tool, users must provide a data file in which each row represents a separate treatment condition and the columns specify the keys (variables) that define the treatment condition (e.g. cell line, drug or other perturbagen, perturbagen concentration, treatment time, replicate) and the measured cell counts (or surrogate such as CellTiter-Glo® or other readout). Analogous traditional metrics: IC50, EC50, Emax, Einf, AUC, and h are also computed. Interactive analysis and visualization tools are provided. Detailed instructions can be found below.
GRcalculator accepts comma-separated (.csv) or tab-separated (.tsv) input files. It can also accept files that use commas as decimal points.
The input files can come in one of two different formats, here called “Case A” and “Case B”. Case A is the simplest format, with treated and control cell counts in different columns. Case B is more general and has treated and control cell counts in the same column. See below for exact descriptions of Case A and Case B.
Click the Load Example button and then click Load Example Case A or Load Example Case B to the left to view a sample data file in the Data Tables tab.
The key variables selected define on which variables the GR values will be averaged before fitting of the dose-response curves. By default, all variables are pre-selected, but, for example, replicates can be averaged by deselecting the proper column header. To deselect a variable, click on it in the grouping variables box and then press the “delete” or “backspace” key. You may repeat this with as many variables as you like.
Additionally, the user may select Advanced options and choose Cap GR values below 1 and/or Force sigmoidal fit. If Cap GR values below 1 is selected, all calculated GR values that are greater than 1 will be set to 1. If Force sigmoidal fit is selected, the calculator will attempt to “force” a sigmoidal rather than a straight line fit, i.e. it will allow for sigmoidal fits with F-test p-values greater than .05.